anti vsv g antibody  (ATCC)

Journal: The Journal of Biological Chemistry

Article Title: Cytosolic nucleic acid sensing triggers type I interferon activation via the Hippo kinase LATS1

doi: 10.1016/j.jbc.2026.111204

Figure Lengend Snippet: Cellular antiviral host defenses are dependent on LATS1. A and B , plaque assay for viral titers from supernatants of WT and Lats1 −/− MEF cells infected with VSV (MOI 0.1) ( A ) or HSV-1 (MOI 0.4) for 16 h. C , immunoblot analysis ( left panel ) and relative quantification ( right panel ) for VSV encoded glycoprotein, G (VSV-G) in WT and Lats1 −/− MEF cells infected with VSV (MOI 0.1) for the indicated times. D , immunoblot analysis ( left panel ) and relative quantification ( right panel ) of HSV-1 encoded immediate early transcription factor, ICP4 (HSV-1 ICP4) expression in WT and Lats1 −/− MEF cells infected with HSV-1 (MOI 0.1) for the indicated times. E and F , fluorescent imaging of WT and Lats1 −/− MEF cells infected with VSV-GFP (MOI 0.1) ( E ) or HSV-1-GFP (MOI 0.1) ( F ) for 24 h. Left to right : bright field, GFP, merged channels. Scale bar = 170 μm. Statistical significance was determined using student’s t test (∗∗∗ p < 0.001, ∗∗ p < 0.01, and ∗ p < 0.05).

Article Snippet: Primary antibodies used in this study were FLAG (Sigma Life Sciences); VSV-G, HSV-1 ICP4, HSP90, P65 (Santa Cruz Biotechnology); TBK1, p-TBK1, IRF3, p-IRF3, LATS1, p-LATS1, p-YAP, YAP/TAZ, STING, p-STING, p-P65, β-Actin (Cell Signaling Technology); β-Tubulin, GAPDH, HA (Proteintech); P65 (Santa Cruz Biotechnology).

Techniques: Plaque Assay, Infection, Western Blot, Quantitative Proteomics, Expressing, Imaging